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Nef controls expression ranges of cell floor CD4 molecules which can be important for adaptive immunity. Despite the shortage of mounted and steady constructions, Nef bodily interacts with the host mobile proteins (AP-1/MHC-I) and modulates intracellular trafficking pathways.
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The Nef insert in pSIVNef-TPA was sequenced, and one change (isoleucine to asparagine) at position 140 was discovered. The pSIVNef-TPA vector has the leader sequence of the human tissue of plasminogen activator protein (TPA) in front of the nef gene, allowing for secretion of the product.
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Current generated from the oxidation of NO is directly proportional to NO concentration, with detection limits of ≈10 nmol/L NO. A selective gas-permeable hydrophobic membrane is used to allow diffusion of NO to the electrode surface while limiting access of NO 2 − and other ionic species. Commercially available electrochemical NO electrodes use Clark-type platinum electrodes (1 to 2 mm ...
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