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This woody tissue was immersed in liquid nitrogen, grinded in a ball-mill (Mixer Mill MM301, Retsch, Germany) and a Qiagen DNeasy Plant Mini Kit was used for DNA extraction. 5 μL of DNA ...
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1 INTRODUCTION. The Chinese rose (Rosa chinensis) is a member of the genus Rosa and is native to south-west China (Lin et al., 2019).Because of its great economic, cultural, and symbolic value, R.chinensis has been widely cultivated around the world (Nybom & Werlemark, 2017).Over 30 fungal pathogens have been reported from R.chinensis (Farr & Rossman, 2021; Xu & He, 2008).
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from leaves and triturated by shaking in a vibratory mill (Mixer Mill MM301, Retsch, Haan, Germany) for 1.5 min at 30 Hz in a 1.5 mL tube together with one tungsten carbide
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Hilden, Germany) on a Retsch MM301 mixer mill ( & Co., Haan, Germany) for 2×2min, first at 18 and then 20 oscillations/s. We eluted the samples twice in 50‐μL elution buffer in separate tubes, one as a back‐up aliquot. We prepared eight 24‐plexed RADseq libraries using the
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Retsch Mm301 Laboratory Mixer Mill 207410001 With Adapter For 10 Test Tubes. For Sale Retsch Mm301 Laboratory Mixer Mill 20.741.0001 With Adapter For 10 Test Tubes, Unit Is Used. Sold by alvin1462 in Willingboro. $3000.00. Mpa-50-mill-es-rg Mts 100 Tested By Dhl Or Ems .
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The mixture was homogenized for 6 min at 24 Hz in a Retsch MM301 mixer mill (ThermoFisher Scientific Inc.) in microtubes containing a 3 mm tungsten carbide bead (the mixer mill rack was rotated 180° halfway through homogenization) to create a standard slurry (Additional file 1: Figure S1).
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Total RNA and genomic DNA was simultaneously extracted from frozen tissue after automated grinding (Retsch Mixer Mill MM301, Haan, Germany) in TRIzol® (Invitrogen, Carlsbad, CA) and chloroform. RNA was purified using RNeasy Midi columns (Qiagen, Valencia, CA) according to manufacturer's instructions and tested for quality and quantity with ...
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An oscillatory ball mill Mixer Mill MM301, Retsch amp Co., Germany was used for cryo milling as follows 1 g of indomethacin was placed into a 25 mL milling jar and milled with two 12 mm balls for 120 minutes. The jars were immersed in liquid nitrogen for 3 min before milling and repeatedly for 3 min at 15 min intervals.
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Fleas of the same species from the same burrow or rodent host were sorted into pools of up to 10 fleas and then homogenized in brain-heart infu- sion broth by using glass beads and Mixer Mill MM301 (Retsch, Haan, Germany). Rodent carcasses were tested at CDPH, CDC, and NPS.
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samples were pulverized using a ball mill (Mixer Mill, MM301; Retsch and Co. Haan, Germany) in order to get very fine and homogeneous plant material. We applied a chemical digestion followed by a colorimetric analysis, as the gravimet-ric approach has been proven to show a poor reproducibility
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Oscillatory Ball Mill Mixermill Mm301 Retsch From India. Processing capacity:79-487t/h Feeding size:≤13mm Appliable Materials: silicate,new-type building material,non-ferrous metal,construction rubbish,cement clinker,ceramics etc.All grindable materials, various metal ores, non-metallic ores, non-flammable and explosive materials
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The mixer mill MM 200 is a compact versatile benchtop unit, which has been developed specially for dry grinding of small amounts of sample. It can mix and homogenize powders in only a few seconds....
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The RETSCH mixer mills MM 200 andMM 301 serve the purpose of fineand ultra-fine grinding of not onlyhard, medium-hard, and brittle sam-ples, but also elastic and fibrous ones. With our mills you can grind: Tissues, bones and hair, Chemicals, drugs, coated and uncoated tablets, Minerals, ores, and alloys, Glass and ceramics, Soils and slurries,
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100 l TRIzol (Life Technologies, Gaithersburg, MD) using a Mixer Mill MM301 (Geneq, Montreal, Canada) at 20-25 Hz. RNA was further isolated and cleaned using RNeasy Mini kit (Qiagen, German-town, MD) according to the manufacturer's instructions. RNA integ-rity, purity and quantity were assessed by Bioanalyzer (Agilent
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Dried leaves were ground in a ball-mill (Mixer Mill MM301; Retsch, Haan, Germany), and DNA extraction was performed with a modified CTAB extraction protocol (Rigueiro et al., 2009 ).
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Productos. Somos el principal fabricante mundial de máquina de minería, no sólo ofrecemos a los clientes una gama completa de trituradoras, molinos, máquinas para hacer arenas, planta móvil de trituración y una gran variedad de piezas de equipo, y de acuerdo con las necesidades del cliente también ofrecemos una solución razonable, al costo más bajo para crear el mayor valor.
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The mixer mill MM 301 is ideally suitable for cryogenic grinding. The sample and grinding ball charge are placed in the screw-top stainless steel grinding jars, which are then immersed in liquid nitrogen. They are fastened in the quick-clamping device of the MM 301; this holds the grinding jars securely, even at extremely low temperatures.
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crushed for 3 min at 30 vibrations/s in an MM301 mixer mill (Retsch). The tube was placed in a 65℃ water bath for 15 min before adding 400 µl chloroform:isoamyl alcohol (24:1). The fungal matrix was spun down for 5 min at 15,521. The supernatant was transferred into g a new centrifuge tube, and ammonium acetate solution (final con-
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then ground in a Retsch Mixer Mill MM301 (Retsch, Hann, Germany) at 30 rps for 90 s. Genomic DNA was extracted Bocas Coco Solo PonSok Mananti G~~~~~ Guacalillo David Figure 1 Distribution of large populations of mangroves in Costa Rica and Panama (adapted from Ellison, 2004) and the five populations of Datronia caperata sampled in this study:
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137 a bead mill (Retsh Mixer Mill MM301) prior to DNA extraction. DNA was extracted with the 138 DNeasy UltraClean® Microbial DNA Isolation Kit (Qiagen) with the addition of Proteinase K 1% 139 to the lysis mix, and a prolonged lysis incubation of 2 hours at 62 ⁰C. DNA was purified with
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Retsch Mixer Mill MM 301. Keith Larson. January 10, 2018. CIRC Climate Chamber Room. Retsch Mixer Mill MM 301. Specifications (see manual for details): Sample volume max.: 2 x 20 ml Feed grain size max.: 8 mm Final fineness down to: < 5 μm normal grinding times: Ø 2 minutes
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After sampling of spectra from whole leaves, all leaves within a cohort were merged and milled into fine powder using a ball mill (Mixer Mill, MM301; Retsch & Co. Haan) and pressed into tablets (Ø 16 mm, 1 mm thick) using a hydraulic press with 4 tonnes of pressure. Finally, we sampled spectra from each tablet.
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Ball Mill Sole Plate This crown should be between .002″ and . 003″, per foot of length of sole plate. For example, if the sole plate is about 8′ long, the crown should be between .016″ and .024″. Ball Mill Sole Plate After all shimming is completed, the sole plate and bases should be grouted in position.
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restsch hammer mill. qiagen tissuelyser retsch mm301 mm 301 mixer mill grinder tissue disruption system. (2 sets of adapter plates and 2 racks for use with 1.5 - 2.0 ml microcentrifuge tubes on the TissueLyser). 2 X 24 We...
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Used Ball Mills. EquipNet is the world's leading global provider of used ball mills and various other preowned equipment. Our exclusive contracts with our clients yield a wide range of used ball mills from a number of respected OEMs, including Fryma Koruma and more. EquipNet is constantly receiving used ball mills of different makes and models.
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Clean. 120V. Includes Qiagen 2 x 24 Well Adapter Sets with Buy It Now QIAGEN TISSUELYSER RETSCH MM301 MIXER MILL GRINDER Tissue Disruption System *** 120V, 50/60 ... $4,690. Vero Beach, FL, USA
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Features of the Retsch MM301 Mixer Mill include: - Reproducible Dry Grinding, - Loss-Free Wet Grinding with Screw-Top, Leak Proof Grinding Jars, - Cryogenic Grinding with Minimal Consumption of Liquid Nitrogen, - Quick, Efficient Pulverization and Homogenization, - Memory Keys for Three Parameter Combinations,
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Legs were removed using clean, sterile forceps and were homogenised using a mixer mill (Retsch Mixer Mill MM301). Total DNA was extracted using the DNeasy blood and tissue kit (Qiagen, Hilden, Germany), according to manufacturer's instructions. The extracted DNA was stored at -20°C until further analysis.
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The physical mixture of IMC-SAC (molar ratio = 1:1) was ground in an oscillatory ball mill (Mixer Mill MM301, Retsch & Co., Germany) with 20 Hz oscillation frequency for 30 min. About 0.2 g mixed powders were placed into a 25-mL-volume stainless-steel milling jar containing two 12-mm-diameter stainless-steel balls by grinding at ambient ...
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QIAGEN TISSUELYSER RETSCH MM301 Mixer Mill Grinder FOR SALE, ← back to all Mill Grinder, QIAGEN TISSUELYSER RETSCH MM301 Mixer Mill Grinder, 4 units left at this price, what is this? 6 people are currently viewing this device. $10,960.65, Pre-Owned (RAW) (-$1,096.07) save 10%, Refurbished, (+$0.00) as standard,
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VFZ-150, CONE MILL. ... الدردشة على الانترنت. Retsch Mixer Mill MM400 100 240 VAC 50 60 Hz from Cole-Parmer. Buy Retsch Mixer Mill MM400 100 240 VAC 50 60 Hz and more from our ... Save to shopping list ... Retsch Mixer Jar Mill Selection Guide.pdf(119.51 KB).
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homogenization for 30 s at 24 Hz in a Mixer Mill MM301 (Retsch, Newtown, PA), followed by centrifu-gationat6000rcffor5 min.WNV-positivepoolswere identified by quantitative real-time reverse transcrip-tion polymerase chain reaction (qRT-PCR) [26]. WNV prevalence was determined using maximum likelihood estimation (MLE) based on mosquito sur-
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Aliquots were Wnely ground with a Mixer Mill (MM301, Retsch, Germany) and organic C and total N contents determined using a Europa ANCA GSL analyzer (PDZEuropa, Crewe, England).
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extraction, the samples were ground in a bead mill (Retsch MM301 Mixer Mill) for 10 min and thereafter stored at −20°C until analysis. In2013,threeoffivesamplingpoints(numbers1,3,5)fromeachfield wereanalyzedseparately(Fig.2,2013)Similarly,insoilsamplesfrom 2014, three of eight samples (numbers 1, 3, 5) from each field were
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Mixer mills MM 200, MM 301 Centrifugal ball mill S 100 Planetary ball mills PM 100, PM 200, PM 400 RETSCH ball mills are used for the pulverization of soft, fibrous, hard and brittle materials. They achieve a very high final fineness down to the submicron range. The feed size depends on the mill and can have a maximum size of 10 mm. If the ...
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G172874 RETSCH MM301 Mixer Mill W/ Operating Instructions Mm 301. Be the first to write a review. About this product. Pre-owned. $1,795.00*. Make an offer: Pre-owned. 3 watching. 7.
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(Qiagen) using a Mixer Mill MM301 (Retsch) at 30 Hz for 2 min. RNA was isolated following the manufac-turer's protocol or the protocol of Wei and Kahn [23]. Purified RNA was dissolved in RNase-free water (Ambion). Concentration was measured using Nano-Drop and RNA quality assayed on an Agilent 2100 BioAnalyzer. The purified RNA was stored at ...
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